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PERMANENT GENETIC RESOURCES: Isolation of microsatellite loci from the kelp, Saccorhiza polyschides (Heterokontophyta, incertae sedis)
Authors:Engel C R  Guillemin M-L  Jacob A-M  Valero M  Viard F
Affiliation:Equipe Evolution et Génétique des Populations Marines, UMR CNRS-UPMC 7144, LIA 'DIAMS', Station Biologique de Roscoff, B.P. 74, Place Georges Teissier, 29682 Roscoff cedex, France, Center for Advanced Studies in Ecology and Biodiversity (CASEB), LIA 'DIAMS', Departameto Ecologia, Casilla 114-D, Pontificia Universidad Católica de Chile, Santiago, Chile.
Abstract:Kelps are ecologically important seaweeds that dominate the subtidal zones of rocky coasts. In Northern Europe, Saccorhiza polyschides is a pioneer species suspected of outcompeting the harvested kelp, Laminaria digitata. To examine how the process of species competition affects species distribution and genetic diversity in coastal environments, we developed 10 polymorphic microsatellite markers for S. polyschides using an enriched library (microsatellites are already available for L. digitata). These loci showed from three to 24 alleles with heterozygosities ranging from 0.36 to 0.92. This polymorphism is high enough for fine-scale population analyses including assignment tests to determine the origin of recruits.
Keywords:brown seaweeds  kelp  microsatellites  North Atlantic  population structure  species distribution
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