Gel-phase P-NMR. A new analytical tool to evaluate solid phase oligonucleoside synthesis.

This paper shows Gel-phase ³¹P-NMR spectra of synthetic intermediates obtained during solid-phase oligonucleotide synthesis on polystyrene for the first time. We have demonstrated the application of this technique using the phosphotriester, H-phosphonate and phosphite triester approaches. The use of Gel-phase ³¹P-NMR for monitoring solid phase oligonucleotide synthesis is discussed.