Evaluation and optimization of factors affecting the performance of a flow-through system based on immobilized acetylcholineesterase as a biosensor |
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Authors: | Bahman Ebrahimi Seyed Abbas Shojaosadati Seyyed Mohammad Mousavi |
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Institution: | (1) College of Environment and Resource, Fuzhou University, 350108 Fuzhou, China |
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Abstract: | A flow-through system based on acetylcholineesterase (AChE) was studied. The system was prepared by mixing AChE and a multiwall
carbon nanotube (MWCNT). Two important parameters, the ratios of AChE:MWCNT (X1) and AChE-MWCNT:sol-gel (X2) were optimized using response surface methodology. The results revealed that an enzyme immobilized within the MWCNT-sol-gel
was more effective compared to one conducted with sol-gel. The optimum feed flow rate was 0.4 mL/min and ATChI concentration
was found to be 1 mM. The optimum ratios of X1 and X2 for immobilization on ceramic packing were 1.07 and 0.43, respectively. The sensitivity of this flow-through system was 1.82
× 10−5/μM and long-term stability analyzed after 120 days was 74% of initial absorbance. With respect to an incubation time of 14
min, the detection limit for paraoxon was 7.3 × 10−12 mol. |
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