A proteomic study of cMyc improvement of CHO culture |
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Authors: | Darrin Kuystermans Michael J Dunn Mohamed Al-Rubeai |
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Institution: | (1) School of Chemical and Bioprocess Engineering, University College Dublin, Belfield Dublin 4, Ireland;(2) UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield Dublin 4, Ireland |
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Abstract: | Background The biopharmaceutical industry requires cell lines to have an optimal proliferation rate and a high integral viable cell number
resulting in a maximum volumetric recombinant protein product titre. Nutrient feeding has been shown to boost cell number
and productivity in fed-batch culture, but cell line engineering is another route one may take to increase these parameters
in the bioreactor. The use of CHO-K1 cells with a c-myc plasmid allowing for over-expressing c-Myc (designated cMycCHO) gives a higher integral viable cell number. In this study
the differential protein expression in cMycCHO is investigated using two-dimensional gel electrophoresis (2-DE) followed by
image analysis to determine the extent of the effect c-Myc has on the cell and the proteins involved to give the new phenotype. |
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