| 拟南芥耐盐突变体stg2的筛选 |
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| 引用本文： | 吕思敏. 拟南芥耐盐突变体stg2的筛选[J]. 氨基酸和生物资源, 2007, 29(1): 25-29. DOI: 10.3969/j.issn.1006-8376.2007.01.008 |
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| 作者姓名： | 吕思敏 |
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| 作者单位： | 武汉大学生命科学学院,武汉,430072 |
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| 摘 要： | 采取在高盐平板上萌发的方法,对一个雌激素诱导激活型拟南芥突变体库进行了耐盐突变体的筛选,最终得到了2株稳定的耐盐突变体。本文中对其中的一株耐盐突变体,命名为stg2(salt tolerance during germination 2),进行了研究。遗传实验表明它的耐盐特性是受雌激素诱导的,是功能获得型的耐盐突变体。本实验中还探讨了stg2突变体的筛选过程及耐盐生理特点。
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| 关 键 词： | 拟南芥 诱导激活型突变体库 耐盐突变体 |
| 文章编号： | 1006-8376(2007)01-0025-05 |
| 修稿时间： | 2006-11-15 |
Screening of Salt - Tolerant Mutant stg2 of Arabidopsis thaliana |
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| LU Si-min. Screening of Salt - Tolerant Mutant stg2 of Arabidopsis thaliana[J]. Amino Acids & Biotic Resources, 2007, 29(1): 25-29. DOI: 10.3969/j.issn.1006-8376.2007.01.008 |
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| Authors: | LU Si-min |
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| Affiliation: | College of Life Science, Wuhan University, Wuhan 430072, China |
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| Abstract: | By seed germinating on salt-containing media,a population of estrogen-inducible activation-tagging Arabidopsis thaliana mutant has been screened for salt-tolerant mutants,and two stable salt-tolerant mutants were obtained.One of them,designatedas stg2(salt tolerance during germination 2) was studied.Genetic analysis showed that its salt tolerance was induced by estrogen,thus it was a functional mutant.The isolation and characterization of stg2 mutant were also investigated. |
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| Keywords: | Arabidopsis thaliana inducible activation-tagging mutant library salt-tolerant mutant |
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