Microcalorimetric studies on the polyhydroxyalkanoates production of recombinant Escherichia Coli |
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| Authors: | L Ruan Y Wang Q Yan P H Yu |
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| Institution: | (1) Open Laboratory of Chirotechnology of the Institute of Molecular Technology for Drug Discovery and Synthesis, Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hong Kong, China |
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| Abstract: | The thermogenic curves of metabolism of two strains of Escherichia coli pUC19cab/XL-IBlue and XL-IBlue have been determined by using a LKB-2277 bioActivity Monitor and ampoule method at 37°C. pUC19cab/XL-IBlue
was a recombinant E. coli strain bearing a foreign plasmid pUC19cab which brought the polyhydroxyalkanoates (PHAs) production. XL-IBlue was a host
bacterium without any foreign DNA. Our studies reveal that the PHA production of recombinant E. coli has an apparent influence on their thermogenic curves of metabolism and therefore the initial time of PHAs production can
be determined from these thermogenic curves.
The text was submitted by the authors in English. |
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