Fluctuation in levels of endogenous plant hormones in ovules of normal and mutant cotton during flowering and their relation to fiber development |
| |
Authors: | J-G Chen X-M Du H-Y Zhao X Zhou |
| |
Institution: | (1) Department of Agronomy, Nanjing Agricultural University, 210095 Nanjing;(2) Division of Scientific Research, Nanjing Agricultural University, 210095 Nanjing;(3) Institute of Cotton, The Chinese Academy of Agricultural Sciences, 455112 Anyang, China |
| |
Abstract: | The contents of indole-3-acetic acid (IAA), gibberellins (GAs), abscisic acid (ABA), and cytokinins were determined in ovules of normal cotton (Tm-1) and a kind of fiber differentiation mutant (Xin) before and after flowering by enzyme-linked immunosorbent assays. It was found that 24 h before flowering, a peak of IAA content was observed in ovules of Tm-1, whereas in ovules of Xin, a low level of IAA was determined. From –1 day (1 day before flowering) to +3 days (3 days after flowering), GA1+3 levels in ovules of Xin were 40–70% lower than those of Tm-1; GA4+7 levels were very low, and there was no visible difference in GA4+7 content between normal and mutant cotton. The ABA content in ovule of Tm-1 decreased by 70% 3 days after flowering, whereas that of Xin only decreased by 20%. The levels of cytokinins in ovules of Tm-1 decreased after flowering, and those of Xin kept up a steady increase.Abbreviations IAA
indole-3-acetic acid
- GA
gibberellin
- ABA
abscisic acid
- ELISA
enzyme-linked immunosorbent assay
- FW
fresh weight
- PBS
phosphate-buffered saline
- iPA
isopentenyladenosine
- ZR
zeatin riboside
- DHZR
dihydrozeatin riboside
- CTK
cytokinin |
| |
Keywords: | Plant hormones Cotton ovule Mutant Fiber development |
本文献已被 SpringerLink 等数据库收录! |
|