Rev binds specifically to a purine loop in the SL1 region of the HIV-1 leader RNA |
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Authors: | Gallego Jose Greatorex Jane Zhang Hui Yang Bin Arunachalam Shyamala Fang Jianhua Seamons John Lea Susan Pomerantz Roger J Lever Andrew M L |
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Institution: | Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom. |
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Abstract: | The leader RNA sequence of human immunodeficiency virus type 1 (HIV-1) consists of a complex series of stem loop structures that are critical for viral replication. Three-dimensional structural analysis by NMR of one of these structures, the SL1 stem loop of the packaging signal region, revealed a highly conserved purine rich loop with a structure nearly identical to the Rev-binding loop of the Rev response element. Using band-shift assays, surface plasmon resonance, and further NMR analysis, we demonstrate that this loop binds Rev. HIV-1 appears to have a second Rev-binding site close to the major splice donor site that may have an additional role in the viral life cycle. |
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