Regional differences in expression of VEGF mRNA in rat gastrocnemius following 1 hr exercise or electrical stimulation |
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Authors: | Tom D Brutsaert Timothy P Gavin Zhenxing Fu Ellen C Breen Kechun Tang Odile Mathieu-Costello and Peter D Wagner |
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Institution: | (1) Department of Physiology, School of Medicine, University of California, San Diego, 92093-0623A, USA |
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Abstract: | Background Vascular endothelial growth factor (VEGF) mRNA levels increase in rat skeletal muscle after a single bout of acute exercise. We assessed regional differences in VEGF165 mRNA levels in rat gastrocnemius muscle using in situ hybridization after inducing upregulation of VEGF by treadmill running (1 hr) or electrical stimulation (1 hr). Muscle functional regions were defined as oxidative (primarily oxidative fibers, I and IIa), or glycolytic (entirely IIb or IId/x fibers). Functional regions were visualized on muscle cross sections that were matched in series to slides processed through in situ hybridization with a VEGF165 probe. A greater upregulation in oxidative regions was hypothesized. |
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