Proteomics-Driven Analysis of Ovine Whey Colostrum |
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| Authors: | Domenica Scumaci Francesca Trimboli Ludovica Dell’Aquila Antonio Concolino Giusi Pappaianni Laura Tammè Giorgio Vignola Alessia Luciani Daniela Morelli Giovanni Cuda Andrea Boari Domenico Britti |
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| Affiliation: | 1Dpt. of Experimental and Clinical Medicine, Magna Græcia University of Catanzaro, Catanzaro, Italy;2Dpt. of Health Science, Magna Græcia University of Catanzaro, Catanzaro, Italy;3Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy;4Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise (IZSAM) “G. Caporale”, Teramo, Italy;Università della Calabria, ITALY |
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| Abstract: | The aim of this study was to shed light in to the complexity of the ovine colostrum proteome, with a specific focus on the low abundance proteins. The ovine colostrum is characterized by a few dominating proteins, as the immunoglobulins, but it also contains less represented protein species, equally important for the correct development of neonates. Ovine colostrum, collected immediately after lambing, was separated by 1D SDS-PAGE. Proteins bands were digested with trypsin and the resulting peptides were analyzed by LC-MS/MS. On the basis of the Swiss-Prot database, a total of 343 unique proteins were identified. To our knowledge, this study represents the most comprehensive analysis of ovine colostrum proteome. |
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