Responsiveness to hormone, growth factor and drug treatment of a human breast cancer cell line: Comparison between early and late cultures |
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Authors: | Alessandra de Cupis Paolo Pirani Laura Fazzuoli Roberto E Favoni |
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Institution: | (1) Department of Preclinical Oncology, National Institute for Cancer Research, Largo R. Benzi 10, 16132 Genoa;(2) Department of Endocrinological and Metabolical Sciences, University of Genoa, Viale Benedetto XV, 10, 16132 Genoa, Italy;(3) Department Preclinical Oncology, Laboratory Pharmacology, Istituto Nazionale per la Ricerca sul Cancro, Largo R. Benzi, 10, 16132 Genoa, Italy |
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Abstract: | Summary Growth rate, morphology, and responsiveness to mitogenic stimuli and pharmacological treatments were evaluated in early and
late cell passages derived from the same clone of the widely used MCF-7 human breast adenocarcinoma cell line. Our results
indicate dissimilarities between early (E) and late (L) passages for some of the parameters analyzed. The cells that underwent
many subcultivations grew faster than the others; both appeared homogeneous in size and shape. The E cells, subcultured for
almost 1 yr, displayed higher sensitivity to the mitogenic action of both estradiol, according to the level of estrogen receptor,
and insulin-like growth factor-I than did the L cells, kept in culture for more than 10 yr. Cell responsiveness to two drugs,
a novel steroid antiestrogen and a polysulfonated distamycin A derivative, was more pronounced in the early cultures only
at the longer time of exposure to the higher concentration of the estrogen antagonist. In addition, a drug-induced inhibition
of insulin-like growth factor-I binding to its receptor was shown in both E and L cells, the latter being less sensitive than
the former when exposed to the antiestrogen. Finally, MCF-7 E and L cells showed similar behavior when drug-induced apoptosis
was tested. |
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Keywords: | breast cancer in vitro cell cultures estrogens growth factors drugs |
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