Advances in Image Correlation Spectroscopy: Measuring Number Densities,Aggregation States,and Dynamics of Fluorescently labeled Macromolecules in Cells |
| |
Authors: | David L Kolin Paul W Wiseman |
| |
Institution: | (1) Department of Chemistry, McGill University, Montreal, QC, Canada, H3A 2K6;(2) Department of Physics, McGill University, Montreal, QC, Canada, H3A 2T8 |
| |
Abstract: | A brief historical outline of fluorescence fluctuation correlation techniques is presented, followed by an in-depth review
of the theory and development of image correlation techniques, including: image correlation spectroscopy (ICS), temporal ICS
(TICS), image cross-correlation spectroscopy (ICCS), spatiotemporal ICS (STICS), k-space ICS (kICS), raster ICS (RICS), and
particle ICS (PICS). These techniques can be applied to analyze image series acquired on commercially available laser scanning
or total internal reflection fluorescence microscopes, and are used to determine the number density, aggregation state, diffusion
coefficient, velocity, and interaction fraction of fluorescently labeled molecules or particles. A comprehensive review of
the application of ICS techniques to a number of systems, including cell adhesion, membrane receptor aggregation and dynamics,
virus particle fusion, and fluorophore photophysics, is presented. |
| |
Keywords: | Image correlation spectroscopy Fluorescence correlation spectroscopy Membrane dynamics Fluorescence microscopy Membrane receptors |
本文献已被 PubMed SpringerLink 等数据库收录! |
|