Binding of dantrolene-Na to the ciliated membrane ofParamecium aurelia |
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Authors: | Shohreh Maleki Shahla Amini |
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Institution: | (1) Pharmaceutical Research Center, Darou-Pakhsh Pharmaceutical Co., Darou-Pahksh Ave., Karaje Road, POBox 13/185-877, Tehran, Iran |
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Abstract: | Summary Dantrolene-Na is a muscular relaxant which binds to sarcoplasmic reticulum (SR) with high affinity and decreases the availability of Ca2+ channels. The binding of fluorescent compounds, dantrolene-Na, nifedipine and chlortetracycline to the ciliary membrane ofParamecium aurelia has been studied. Dantrolene at the concentrations of 1.9 · 10–5, 3.8 · 10–5 and 7.9 · 10–5 M manifested a punctuated binding pattern to the cell membrane. Isolated cilia also bound dantrolene at their basal portion, whereas deciliated cell bodies lost their dotted binding pattern. Chlortetracycline showed a similar but weaker fluorescent staining. Nifedipine treated cells revealed no sign of fluorescent binding to the membrane and was only taken up in food vacuoles.Based on these observations we propose that dantrolene binding regular arrays ofParamecium cell membrane could be identical to granular plaques observed by electron microscope. The possible functioning of these structures as Ca2+ reservoirs is also discussed. |
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Keywords: | Calcium Chlortetracycline Dantrolene-Na Nifedipine Paramecium |
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