Evaluation of reductive amperometric detection in the liquid chromatographic determination of antineoplastic platinum complexes |
| |
Authors: | Steve J Bannister Larry A Sternson A J Repta |
| |
Institution: | Pharmaceutical Chemistry Department, The University of Kansas,Lawrence, KS 66045 U.S.A. |
| |
Abstract: | The usefulness of reductive electrochemical detection at mercury drop electrodes has been determined for platinum complexes separated by solvent-generated anion-exchange high-performance liquid chromatography. Both current-sampled dropping mercury and hanging mercury drop electrodes (DME and HMDE) provide significant advantages over UV absorbance and off-line non-flame atomic absorption detection. The effects of chromatographic and polarographic parameters on analytical system performance have been investigated. By raising the detector cell temperature, the detector response to cis-dichlorodiammineplatinum(II) (DDP) can be shifted anodically to 0.0 V vs. Ag/AgCl, thereby increasing detector selectivity for this compound. The noise-limited minimum detectable quantities of DDP with DME and HMDE are 1.8 ng and 70 pg injected, respectively. DDP can be determined in untreated urine at levels below 100 ng/ml. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|