氧化修饰HDL对培养人主动脉平滑肌细胞胆固醇流出的影响

大量研究显示,高密度脂蛋白(highdensitylipoprotein,HDL)具有抗动脉粥样硬化(atherosclerosis,AS)作用.这是由于HDL能够促进外周组织如血管壁内皮细胞、平滑肌细胞(smoothmusclecell,SMC)及巨噬细胞储集的胆固醇流出,并将其转移到肝脏通过胆汁分泌而排出体外1],这一过程...

Effect on Cholesterol Effluxing Capacity from Cultured Human Arterial Smooth Muscle Cells by Oxidized High Density Lipoprotein

Intracellular accumulation of cholesterol in vascular wall cells is critical in atherosclerosis. HDL causes cholesterol effluxing from endothelial cells,macrophages and fibroblasts. To study the effect on cholesterol effluxing from cultured human arterial smooth muscle cells (SMC) by oxidized HDL (OX HDL),effect of native HDL(N HDL) and OX HDL on 3 H cholesterol effluxing from cultured SMC by loading cultured human arterial SMC with 3 H cholesterol was investigated. The results showed that 3 H cholesterol effluxing capacity from cultured SMC was 44 6% after incubating 24 hours with N HDL and was not increased by further incubating 48 and 72 hours with N HDL. 3 H cholesterol effluxing capacity from SMC decreased significantly after SMC incubating 24, 48 and 72 hours with OX HDL compared with N HDL(14 6% vs 44 6%,23 3% vs 44 2%,30 4% vs 43 2% respectively, all P <0 01).The results suggest that OX HDL decrease significantly 3 H cholesterol effluxing from cultured human SMC and may play an important role in atherosclerosis.