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| 阪崎肠杆菌核酸提取纯化方法分析比较 | |
| 引用本文： | 刘张岚,王晶,石磊,黄晓蓉,张玲. 阪崎肠杆菌核酸提取纯化方法分析比较[J]. 生物技术通报, 2010, 0(4) |
| 作者姓名： | 刘张岚 王晶 石磊 黄晓蓉 张玲 |
| 作者单位： | 1. 中国计量科学研究院生物能源与环境研究所,北京,100013 2. 华南理工大学食品学院,广州,510640 3. 福建省出入境检验检疫局,福州,350001 |
| 基金项目： | "十一五"国家科技支撑计划项目 |
| 摘 要： | 阪崎肠杆菌是一种食源性致病菌,目前越来越多的分子检测技术用于该菌的检测,以取代传统的检测技术。针对分子检测技术相应的核酸标准物质的研制势在必行。核酸的提取和纯化是核酸标准物质的研制过程中的重要环节之一,快速高效高质量,低毒低成本已成为核酸提取的重要目标。就现有方法进行分析比较,重点对常用的三种提取微生物基因组DNA的试剂盒进行了全方位比较,获得了阪崎肠杆菌较优的基因组DNA提取方法,并对提取的DNA进行PCR特异性扩增检测,获得较清晰的谱带。为阪崎肠杆菌核酸标准物质的研制奠定了基础。 |
| 关 键 词： | 阪崎肠杆菌 DNA提取纯化 方法比较 标准物质 |
Comparing of Nucleic Acid Extraction and Purification Methods for Enterobacter sakazakii |
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| Liu Zhanglan,Wang Jing,Shi Lei,Huang Xiaorong,Zhang Ling. Comparing of Nucleic Acid Extraction and Purification Methods for Enterobacter sakazakii[J]. Biotechnology Bulletin, 2010, 0(4) | |
| Authors: | Liu Zhanglan Wang Jing Shi Lei Huang Xiaorong Zhang Ling |
| Affiliation: | Liu Zhanglan1Wang Jing1 Shi Lei2 Huang Xiaorong3 Zhang Ling1(1National Institute of Metrology,Beijing 100013,2South China University of Technology,Guangdong 510640,3Fujian Entry-Exit Inspection & Quarantine Bureau of P.R.C,Fuzhou 350001) |
| Abstract: | Enterobacter sakazakii is a foodborne pathogen.Currently,there are a growing number of molecular detection techniques for the detection of Enterobacter sakazakii in order to replace the traditional detection techniques.To develop the corresponding nucleic acid reference materials is imperative for the molecular detection technology.Nucleic acid extraction and purification is a key link of development in nucleic acid reference materials.With characteristic of rapid,efficient,high-quality,low toxicity and low... |
| Keywords: | Enterobacter sakazakii DNA extraction and purification Methods comparing Nucleic acid reference materials |
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