Adsorption of cytochrome c to phospholipid monolayers studied by reflection spectroscopy |
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| Authors: | Z Kozarac A Dhathathreyan D M?bius |
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| Institution: | Center for Marine Research, Rudjer Boskovic Institute, Zagreb, Croatia, Yugoslavia. |
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| Abstract: | The uptake of cytochrome c by charged and neutral lipid monolayers was studied by using reflection spectroscopy. The method was shown to be a very sensitive and useful technique in studies of lipid-protein interactions. It was found that cytochrome c is preferentially bound to monolayers of negatively charged monolayers in the solid phase. Polarized light under oblique incidence was used to determine the average orientation of chromophores in cytochrome c bound to lipid monolayer. The transition moments of chromophore are oriented parallel to the monolayer plane. |
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