The structure of a fucose-containingO-glycosidic carbohydrate chain of human platelet glycocalicin |
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| Authors: | Sophia A M Korrel Kenneth J Clemetson Herman Van Halbeek Johannis P Kamerling Jan J Sixma Johannes F G Vliegenthart |
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| Institution: | (1) Department of Bio-Organic Chemistry, University of Utrecht, Croesestraat 79, NL-3522 AD Utrecht, The Netherlands;(2) Theodor Kocher Institute, University of Bern, Freiestrasse 1, Postfach 99, CH-3000 Bern 9, Switzerland;(3) Department of Haematology, University of Utrecht, Catharijnesingel 101, NL-3511 GV Utrecht, The Netherlands |
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| Abstract: | The hydrazinolysis procedure currently used for the release ofN-glycosidic carbohydrate chains was applied to glycocalicin. The resulting mixture of oligosaccharide-alditols was fractionated by high-voltage paper electrophoresis into a neutral (5%) and several acidic fractions. The neutral compounds were passed over Bio-Gel P-4. SomeN-glycosidic oligosaccharide-alditols, of theN-acetyllactosamine type as well as of the oligomannoside type, were found to be present. However, oligosaccharide-alditols derived fromO-glycosidic carbohydrate chains were also found, indicating a partial cleavage of GalNAc1-OSer/Thr linkages under the hydrazinolysis conditions applied. One of the neutralO-glycosidic components was characterized, by 500-MHz1H-NMR spectroscopy in combination with sugar analysis, as the following pentasaccharidealditol:
In addition the afuco analogue of this compound was obtained. |
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| Keywords: | glycocalicin platelet membrane O-glycosidic carbohydrate |
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