Effect of Bacteria on Survival and Growth of Acanthamoeba castellanii |
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Authors: | Xiaohui Wang Donald G. Ahearn |
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Affiliation: | (1) Georgia State University, Biology Department, P.O. Box 4010, Atlanta, GA 30303-4010, USA , US |
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Abstract: | The growth and survival of Acanthamoeba castellanii in the presence of Gram-negative bacteria such as Pseudomonas aeruginosa, Escherichia coli, Serratia marcescens, and Stenotrophomonas maltophilia varied with the densities and species of bacteria. All species of bacteria suspended in a buffered saline at densities of 105 to 106/ml supported the growth and survival of 106/ml trophozoites of Acanthamoeba castellanii in a buffered saline solution. At densities of bacteria to amoebae of 100:1 or greater, growth and survival of A. castellanii were suppressed, particularly by P. aeruginosa. In an enrichment medium, the rapid growth of most co-inoculated bacteria inhibited the growth and survival of the amoeba. Received: 12 September 1996 / Accepted: 20 September 1996 |
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