Neuroprotective Effect of Baicalin in a Rat Model of Permanent Focal Cerebral Ischemia |
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Authors: | Xian-kun Tu Wei-zhong Yang Song-sheng Shi Chun-hua Wang Chun-mei Chen |
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Institution: | (1) Department of Neurosurgery, The Affiliated Union Hospital of Fujian Medical University, 29# Xinquan Road, 350001 Fuzhou, Fujian, China |
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Abstract: | This investigation was performed to determine the neuroprotective effect of baicalin on permanent cerebral ischemia injury
in rats and the potential mechanisms in this process. Adult male Sprague-Dawley rats underwent permanent middle cerebral artery
occlusion (pMCAO). The rats were then received intraperitoneal injection with baicalin (10, 30 and 100 mg/kg) or vehicle.
Morphological characteristic, neurological deficit scores, cerebral infarct volume and the enzymatic activity of myeloperoxidase
(MPO) were measured 24 h after pMCAO. The mRNA expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2)
were determined by RT-PCR. Neuronal apoptosis was determined by TUNEL staining and Western blot. Baicalin (30 and 100 mg/kg)
reduced neurological deficit scores and cerebral infarct volume 24 h after pMCAO. Baicalin significantly decreased the enzymatic
activity of MPO and the expression of iNOS mRNA and COX-2 mRNA in rat brain, it also significantly inhibited neuronal apoptosis
and the expression of cleaved caspase-3 protein after pMCAO. Our results suggested that baicalin possesses potent anti-inflammatory
and anti-apoptotic properties and attenuates cerebral ischemia injury. This protection might be associated with the downregulated
expression of iNOS mRNA, COX-2 mRNA, and cleaved caspase-3 protein. |
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Keywords: | Apoptosis Baicalin Cerebral ischemia Inflammation Neuroprotection Stroke |
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