Time-resolved fluoroimmunoassay as a method for detection of Erwinia chrysanthemi in potato peel extracts |
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Authors: | J.M. van der Wolf |
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Affiliation: | DLO Research Institute for Plant Protection (IPO-DLO), Wageningen, The Netherlands |
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Abstract: | J.M. VAN DER WOLF. 1993. Time-resolved fluoroimmunoassay (TR-FIA) was compared with double antibody sandwich (DAS)-ELISA for detection of Erwinia chrysanthemi antigens in potato peel extracts. Pure cultures were used to optimize TR-FIA with respect to the microplate washing procedure and dilution buffer compositions. The detection threshold level for spiked potato peel extracts with the optimized TR-FIA format was 105 cells ml-1 as for the detection level of DAS-ELISA. The signal to background ratios at concentrations above 105 cells ml-1 were higher with TR-FIA than with DAS-ELISA. The dynamic range of TR-FIA was also superior to that of DAS-ELISA. It can be concluded that TR-FIA is an attractive alternative to DAS-ELISA as a detection method for Erw. chrysanthemi, especially when quantification is required. |
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