|
|||||
|
|
Application of enzyme flow microcalorimetry to the study of microkinetic properties of immobilized biocatalyst |
|
| Authors: | V Štefuca A Welwardová P Gemeiner A Jakubová |
| Institution: | (1) Institut de Recherche et de Transfert sur les Lipides, Av. Général Leclerc, 35000 Rennes, France;(2) Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 842 38 Bratislava, Slovakia;(3) Biotika Pharmaceutical Plant, 976 13 Slovenská Lup a, Slovakia |
| Abstract: | Summary Flow microcalorimeter was used for the study of microkinetic properties of Escherichia coli cells enriched with the penicillin G acylase activity immobilized in calcium pectate gel. The experimental kinetic data were obtained by measurement of the thermometric signal in the microcalorimetric column with immobilized enzyme and described by the introduced mathematical model involving the mass transfer and reaction kinetic phenomena. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |