The Feulgen reaction 75 years on |
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| Authors: | Pasquale Chieco M Derenzini |
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| Institution: | (1) Istituto di Oncologia ”F. Addarii”, Policlinico S.Orsola, Viale Ercolani 4/2, I-40138 Bologna, Italy, IT;(2) Dipartimento di Patologia Sperimentale, Universita’ degli Studi di Bologna, Servizio di Citopatologia, Policlinico S.Orsola, Via Massarenti 9, I-40138 Bologna, Italy e-mail: derenzin@med.unibo.it Tel.: +39-051-306861, Fax: +39-051-306861, IT |
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| Abstract: | The Feulgen reaction proposed by Feulgen and Rossenbeck 75 years ago is one of the cytohistochemical reactions most widely
used in biology and medicine. It allows DNA in situ to be specifically stained based on the reaction of Schiff or Schiff-like
reagents with aldehyde groups engendered in the deoxyribose molecules by HCl hydrolysis. The staining intensity is proportional
to the DNA concentration. Current applications of the Feulgen reaction are mainly concerned with DNA quantification in cell
nuclei by image cytometry for ploidy evaluation in tumor pathology. From the morphological point of view, specific demonstration
of DNA in cell structures at the light microscopic level is very little used nowadays. On the other hand, application of the
Feulgen principles to electron microscopy have recently allowed specific DNA-staining procedures to be developed for the study
of the structural organization of DNA in situ.
Accepted: 13 January 1999 |
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