Production enhancement of Rhizopus arrhizus lipase by feeding oleic acid |
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Authors: | Dan Li Bingwu Wang Tianwei Tan |
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Affiliation: | aBeijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 10029, PR China |
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Abstract: | A strategy for Rhizopus arrhizus lipase production enhancement by feeding oleic acid was developed. The oleic acid was proved to have strong inducing effect on lipase production, but high concentration oleic acid could repress lipase production. The decrease rate of oleic acid concentration using peanut oil as initial carbon source was figured out according to the change of oleic acid concentration in the fermentation broth. Our feeding strategy designed based on the decrease rate of oleic acid could avoid the repression of lipase production that is caused by high concentration of oleic acid in the fermenting liquor, and this strategy worked as a new feeding method showing excellent performance. The maximum lipase activity was gained by feeding dilute oleic acid every 12 h starting at 60 h, which maintained the oleic acid concentration around 18 mg/L, and the lipase activity was 31% higher than that of no feeding. |
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Keywords: | Rhizopus arrhizus Feeding strategy Lipase Fermentation Oleic acid |
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