Cyclic AMP-dependent cell shape changes induced by mechanical forces |
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Authors: | Chotard-Ghodsnia R Drochon A Faucheux N Nagel M-D Grebe R |
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Institution: | UMR 6600 CNRS, Biomécanique et Génie Biomédical, Université de Technologie de Compiègne, BP 20529, 60205 Compiègne cedex, France. roxana.ghodsnia@utc.fr |
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Abstract: | Biomaterials used in some biomedical devices are exposed to flow of physiological fluids. The flow-induced forces may influence the morphological and the biochemical responses of adhering cells. The objective of this work is to examine the capacity of a mechanical stress to cause changes in cell/substratum and cell/cell interactions via the second messenger cAMP pathway (cyclic Adenosine Monophosphate). Cyclic AMP is known to modulate cell shape, cell adhesion and intercellular communication in static conditions. A specially designed flow chamber was used to analyze the responses of mouse 3T3 fibroblasts spread on biocompatible substrata and submitted to controlled shear stresses. A 1.1-Pa shear stress induced: cell rounding, disruption of vitronectin receptors clusters and clustering of connexins 43 at cell-cell apposition points. These cell responses were cAMP-dependent. These investigations should help provide a better understanding of the early biochemical events triggered by mechanical forces. |
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