Untargeted mutagenesis induced by UV in the lacI gene of Escherichia coli |
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| Authors: | Roshan B. Christensen J. R. Christensen Ian Koenig Christopher W. Lawrence |
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| Affiliation: | (1) Department of Radiation Biology and Biophysics, University of Rochester School of Medicine and Dentistry, 14642 Rochester, NY, USA;(2) Department of Microbiology, University of Rochester School of Medicine and Dentistry, 14642 Rochester, NY, USA |
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| Abstract: | Summary Using a nonselective method, we have estimated the proportion of untargeted mutations in the lacI gene of E. coli by transferring either irradiated or unirradiated F pro lac plasmids from an excision deficient donor to an excision deficient pro lac deleted recipient that had been irradiated and allowed to induce recA dependent functions for 30 min. We find that about 10 percent of the mutations induced by either 3.5 Jm-2 or 7 Jm-2 UV are untargeted. |
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