Virulence of Paracoccidioides brasiliensis: The influence of in vitro passage and storage |
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Authors: | Elmer Brummer Angela Restrepo Linda H Hanson David A Stevens |
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Institution: | (1) Division of Infectious Diseases, Department of Medicine, Santa Clara Valley Medical Center and Institute for Medical Research, 95128 San Jose, CA, USA;(2) Division of Infectious Diseases, Department of Medicine, Stanford University Medical School, 94305 Stanford, CA, USA;(3) Corporacion de Investigaciones Biologicas, Hospital Pablo Tobon Uribe, Medellin, Colombia |
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Abstract: | Stability of virulence in P. brasiliensis isolates was studied with respect to the in vitro culture history and methods used for storage. Virulence in yeast-form P. brasiliensis isolates was tested in a chronic pulmonary murine model of paracoccidioidomycosis where progression of disease was quantitated in terms of colony forming units recoverable from lungs. Four isolates of P. brasiliensis, including recently isolated from patients or experimental animals, caused chronic progressive disease. Two isolates with a history of subculturing showed attenuation by causing resolving but chronic disease. An attenuated isolate became avirulent subsequent to 15 more years of subculturing. These findings suggest that virulence of P. brasiliensis can be attenuated or lost subsequent to cycles of subculturing over long periods. Our data suggest that the use of fresh P. brasiliensis isolates may be needed to provide reproducible virulence for experimental systems.Abbreviations ATCC
American Type Culture Collection
- CFU
colony-forming units
- LD
Lethal dose
- MMv-M
modified McVeigh Morton
- PMN
polymorphonuclear neutrophil |
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Keywords: | Paracoccidioides brasiliensis paracoccidioidomycosis passage in vitro virulence |
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