Ultrastructural studies of the binding of concanavalin A to the plasmalemma of higher plant protoplasts |
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Authors: | J Burgess P J Linstead |
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Institution: | (1) John Innes Institute, Colney Lane, NR4 7UH Norwich, UK |
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Abstract: | Summary The binding of concanavalin A to the plasmalemma of higher plants has been studied using protoplasts of two species. The lectin aggregates both tobacco (Nicotiana tabacum L.) leaf protoplasts and protoplasts prepared from a suspension cell culture of grapevine (Vitis vinifera L.). Differences in lectin binding have been investigated using concanavalin A conjugated to ferritin or bound to colloidal gold. Tobacco protoplasts exhibit continuous and saturated labelling of the plasmalemma surface with gold-concanavalin A mixtures. Vine protoplasts under the same conditions show a discontinuous and patchy distribution of label. These results are discussed in terms of a possible binding mechanism.Abbreviations ConA
concanavalin A
- PBS
Phospholi Buffered Saline
- PEG
polyethylene glycol |
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