A whole-genome scan for recurrent airway obstruction in Warmblood sport horses indicates two positional candidate regions |
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Authors: | June E Swinburne Helen Bogle Jolanta Klukowska-Rötzler Michaela Drögemüller Tosso Leeb Elizabeth Temperton Gaudenz Dolf Vincent Gerber |
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Institution: | (1) Animal Health Trust, Newmarket, Suffolk, CB8 7UU, UK;(2) Centre for Preventive Medicine, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk, CB8 7UU, UK;(3) Equine Clinic, Department of Veterinary Clinical Studies, Vetsuisse-Faculty, University of Berne, Langgass-strasse 124, 3012 Berne, Switzerland;(4) Institute of Genetics, Vetsuisse-Faculty, University of Berne, Bremgartenstrasse 109A, 3001 Berne, Switzerland |
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Abstract: | Recurrent airway obstruction (RAO), or heaves, is a naturally occurring asthma-like disease that is related to sensitisation
and exposure to mouldy hay and has a familial basis with a complex mode of inheritance. A genome-wide scanning approach using
two half-sibling families was taken in order to locate the chromosome regions that contribute to the inherited component of
this condition in these families. Initially, a panel of 250 microsatellite markers, which were chosen as a well-spaced, polymorphic
selection covering the 31 equine autosomes, was used to genotype the two half-sibling families, which comprised in total 239
Warmblood horses. Subsequently, supplementary markers were added for a total of 315 genotyped markers. Each half-sibling family
is focused around a severely RAO-affected stallion, and the phenotype of each individual was assessed for RAO and related
signs, namely, breathing effort at rest, breathing effort at work, coughing, and nasal discharge, using an owner-based questionnaire.
Analysis using a regression method for half-sibling family structures was performed using RAO and each of the composite clinical
signs separately; two chromosome regions (on ECA13 and ECA15) showed a genome-wide significant association with RAO at P < 0.05. An additional 11 chromosome regions showed a more modest association. This is the first publication that describes
the mapping of genetic loci involved in RAO. Several candidate genes are located in these regions, a number of which are interleukins.
These are important signalling molecules that are intricately involved in the control of the immune response and are therefore
good positional candidates. |
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