High fat feeding and dietary <Emphasis Type="SmallCaps">l</Emphasis>-arginine supplementation differentially regulate gene expression in rat white adipose tissue |
| |
Authors: | Wenjuan Jobgen Wenjiang J Fu Haijun Gao Peng Li Cynthia J Meininger Stephen B Smith Thomas E Spencer Guoyao Wu |
| |
Institution: | (1) Department of Animal Science, Faculty of Nutrition, Texas A&M University, College Station, TX 77843, USA;(2) Department of Epidemiology, Michigan State University, East Lansing, MI 48824, USA;(3) Department of Systems Biology and Translational Medicine, Texas A&M Health Science Center, Temple, TX 76504, USA; |
| |
Abstract: | Dietary l-arginine (Arg) supplementation reduces white-fat gain in diet-induced obese rats but the underlying mechanisms are unknown.
This study tested the hypothesis that Arg treatment affects expression of genes related to lipid metabolism in adipose tissue.
Four-week-old male Sprague–Dawley rats were fed a low-fat (LF) or high-fat (HF) diet for 15 weeks. Thereafter, lean or obese
rats continued to be fed their same respective diets and received drinking water containing 1.51% Arg–HCl or 2.55% l-alanine (isonitrogenous control). After 12 weeks of Arg supplementation, rats were euthanized to obtain retroperitoneal adipose
tissue for analyzing global changes in gene expression by microarray. The results were confirmed by RT-PCR analysis. HF feeding
decreased mRNA levels for lipogenic enzymes, AMP-activated protein kinase, glucose transporters, heme oxygenase 3, glutathione
synthetase, superoxide dismutase 3, peroxiredoxin 5, glutathione peroxidase 3, and stress-induced protein, while increasing
expression of carboxypeptidase-A, peroxisome proliferator activated receptor (PPAR)-α, caspase 2, caveolin 3, and diacylglycerol
kinase. In contrast, Arg supplementation reduced mRNA levels for fatty acid binding protein 1, glycogenin, protein phosphates
1B, caspases 1 and 2, and hepatic lipase, but increased expression of PPARγ, heme oxygenase 3, glutathione synthetase, insulin-like
growth factor II, sphingosine-1-phosphate receptor, and stress-induced protein. Biochemical analysis revealed oxidative stress
in white adipose tissue of HF-fed rats, which was prevented by Arg supplementation. Collectively, these results indicate that
HF diet and Arg supplementation differentially regulate gene expression to affect energy-substrate oxidation, redox state,
fat accretion, and adipocyte differentiation in adipose tissue. Our findings provide a molecular mechanism to explain a beneficial
effect of Arg on ameliorating diet-induced obesity in mammals. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|