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Heterologous transformation ofClaviceps purpurea
Authors:Aleksandra Comino  Margareta Kolar  Helmut Schwab  Helena Sočič
Institution:(1) Institute of Chemistry, Boris Kidriccaron, Hajdrihova 19, Ljubljana, Yugoslavia;(2) Institut fur Biotechnologie, Schlogelgasse 9, A-8010 Graz, Austria
Abstract:Summary A transformation system based on dominant selection markers was established for an industrialClaviceps purpurea strain. Transformants could be obtained by using plasmid pAN 7-1 carrying a bacterial gene for hygromycin (hph) resistance fused to a fungal promoter or by plasmid p3SR2 which carries the acetamidase gene (amdS) fromAspergillus nidulans.
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