Effect of nordihydroguaiaretic acid on intracellular Ca concentrations in C6 glioma cells |
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Authors: | Warren Su Li-Ling Tseng Muh-Chiou Lin Hsin-Ju Chang Kam-Chung Lee Kang-Ju Chou Yuk-Keung Lo Jin-Shiung Cheng Hong-Tai Chang Jue-Long Wang Chun-Peng Liu Wei-Chung Chen Chung-Ren Jan |
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Institution: | Department of Pediatrics, Pao-Chien General Hospital, Ping Tung, Taiwan. |
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Abstract: | The effect of nordihydroguaiaretic acid (NDGA) on Ca(2+) signaling in C6 glioma cells has been investigated. NDGA (5-100 microM) increased Ca(2+)]i concentration-dependently. The Ca(2+)]i increase comprised an initial rise and an elevated phase over a time period of 4 min. Removal of extracellular Ca(2+) reduced NDGA-induced Ca(2+)]i signals by 52+/-2%. After incubation of cells with NDGA in Ca(2+)-free medium for 4 min, addition of 3 mM CaCl2 induced a concentration-dependent increase in Ca(2+)]i. NDGA (100 microM)-induced Ca(2+)]i increases in Ca(2+)-containing medium was not changed by pretreatment with 10 microM nifedipine or verapamil. In Ca(2+)-free medium, pretreatment with the endoplasmic reticulum Ca(2+) pump inhibitor thapsigargin (1 microM) abolished 100 microM NDGA-induced Ca(2+)]i increases. Inhibition of phospholipase C with 2 microM U73122 had little effect on 100 microM NDGA-induced Ca(2+) release. Several other lipoxygenase inhibitors had no effect on basal Ca(2+)]i. Collectively, the results suggest that NDGA increased Ca(2+)]i in glioma cells in a lipoxygenase-independent manner, by releasing Ca(2+) from the endoplasmic reticulum in a manner independent of phospholipase C activity and by causing Ca(2+) influx. |
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Keywords: | C6 glioma cells Ca2+ signaling Ca2+ stores Fura-2 Nordihydroguaiaretic acid (NDGA) |
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