Differentiation of Neuroblastoma Cell Line N1E-115 Involves Several Signaling Cascades |
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Authors: | Ji-eun?Oh Karlin?Raja?Karlmark Joo-ho?Shin Arnold?Pollak Angelika?Freilinger Markus?Hengstschl?ger Email author" target="_blank">Gert?LubecEmail author |
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Institution: | (1) CChem, FRSC (UK), Department of Pediatrics, Medical University of Vienna, Währinger Gürtel 18, A 1090 Vienna, Austria;(2) Obstetrics and Gynecology, Prenatal Diagnosis and Therapy, Medical University of Vienna, Vienna, Austria |
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Abstract: | No systematic searches for differential expression of signaling proteins (SP) in undifferentiated vs. differentiated cell lineages were published and herein we used protein profiling for this purpose. The N1E-115 cell line was cultivated and an aliquot was differentiated with dimethylsulfoxide (DMSO), that is known to lead to a neuronal phenotype. Cell lysates were prepared, run on two-dimensional gel electrophoresis followed by MALDI-TOF-TOF identification of proteins and maps of identified SPs were generated. Seven SPs were comparable, 27 SPs: GTP-binding/Ras-related proteins, kinases, growth factors, calcium binding proteins, phosphatase-related proteins were observed in differentiated N1E-115 cells and eight SPs of the groups mentioned above were observed in undifferentiated cells only. Switching-on/off of several individual SPs from different signaling cascades during the differentiation process is a key to understand mechanisms involved. The findings reported herein are challenging in vitro and in vivo studies to confirm a functional role for deranged SPs. |
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Keywords: | Signaling cascades GTP-binding/Ras-related proteins kinases growth factors calcium binding proteins phosphatase NO signaling proteins retinoic acid-binding protein differentiation N1E-115 two-dimensional gel electrophoresis matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) |
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