Sizing of theRhodococcus sp. R312 genome by pulsed-field gel electrophoresis. Localization of genes involved in nitrile degradation |
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| Authors: | Frédéric Bigey Guilhem Janbon Alain Arnaud Pierre Galzy |
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| Institution: | (1) Chaire de Microbiologie Industrielle et de Génétique des Micro-organismes, E.N.S.A. / I.N.R.A. 2, Place Viala, 34060 Montpellier Cedex 1, France |
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| Abstract: | The two restriction enzymesAsnI andDraI were found to produce DNA fragment sizes that could be used for mapping theRhodococcus sp. R312 (formerlyBrevibacterium sp. R312) genome by pulsed-field gel electrophoresis.AsnI produced 24 fragments (4 to 727 kb) andDraI yielded 15 fragments (8.5 to 2400 kb). The fragment lengths in each digest were summed, indicating that the size of the chromosome ranged from 6.31 to 6.56 Mb, with a mean of 6.44 Mb. In addition, the wide-spectrum amidase gene (amiE) and the operon containing the enantiomer-selective amidase gene (amdA) and the nitrile hydratase structural gene (nthA, nthB) were localized on theAsnI andDraI fragments. |
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| Keywords: | amidase genome size nitrile hydratase pulsed-field gel electrophoresis Rhodococcus |
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