Differences in Doublecortin Immunoreactivity and Protein Levels in the Hippocampal Dentate Gyrus Between Adult and Aged Dogs |
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| Authors: | In Koo Hwang Ki-Yeon Yoo Hua Li Jung Hoon Choi Young-Guen Kwon Youngkeun Ahn In Se Lee Moo Ho Won |
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| Institution: | (1) Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea;(2) Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chuncheon, 200-702, South Korea;(3) Department of Biochemistry, College of Sciences, Yonsei University, Seoul, 120-749, South Korea;(4) The Heart Center of Chonnam National University Hospital 8 Hak Dong, Dong Ku, Gwangju, 501-757, South Korea |
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| Abstract: | Doublecortin (DCX), a microtubule-associated protein, specifically expresses in neuronal precursors. This protein has been
used as a marker for neuronal precursors and neurogenesis. In the present study, we observed differences in DCX immunoreactivity
and its protein levels in the hippocampal dentate gyrus between adult and aged dogs. In the adult dog, DCX immunoreactive
cells with well-stained processes were detected in the subgranular zone of the dentate gyrus. Numbers of DCX immunoreactive
cells in the dentate gyrus of the aged dog were significantly decreased compared to those in the adult dog. DCX immunoreactive
cells in both adult and aged dog did not show NeuN (a marker for mature neurons) immunoreactivity. NeuN immunoreactivity in
the aged dog was poor compared to that in the adult dog. DCX protein level in the aged dentate gyrus was decreased by 80%
compared to that in the adult dog. These results suggest that the reduction of DCX in the aged hippocampal dentate gyrus may
be involved in some neural deficits related to the hippocampus. |
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| Keywords: | Aging Dog Dentate gyrus Neurogenesis Doublecortin NeuN |
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