New isolate of Streptomyces sp. with novel thermoalkalotolerant cellulases |
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Authors: | Faiez Alani William A. Anderson Murray Moo-Young |
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Affiliation: | (1) Biochemical Engineering Group, Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada, N2L 3G1 |
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Abstract: | A Streptomyces sp. was isolated that produced novel thermoalkalotolerant cellulase activity after growth on crystalline cellulose at 50°C. Three major components of the cellulases (CMCase, Avicelase and cellobiase) were produced with maximal activities (11.8, 7.8 and 3.9 IU/ml) and maximum specific activities 357, 276 and 118 IU/mg protein, respectively, after 120 h growth. Maximum CMCase activity was between 50 and 60°C measured over 3 h. The enzyme also retained 88% of its maximum activity at 70°C and pH 5, and 80% of the activity at pH 10 and 50°C when assayed after 1 h. After incubation at 40°C for 1 h with commercial detergent (Tide) at pH 11, 95% activity was retained. The enzyme mixture produced glucose from crystalline cellulose. |
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Keywords: | Avicelase Cellobiase CMCase Streptomyces sp |
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