Structural characteristics of native and enzymically formed dextran of S. sanguis ATCC 10558. |
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Authors: | A T Arnett R M Mayer |
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Institution: | The Ohio State University, Department of Chemistry, Columbus, Ohio 43210 U. S. A. |
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Abstract: | The structure of the native dextran produced by Streptococcus sanguis ATCC 10558 was analyzed by g.l.c.-m.s. of the methylated alditol acetates derived from the polymer. The results indicate that the polymer contains D-glucosyl residues substituted at C-6 or C-3, or both, as well as unsubstituted D-glucosyl residues. These data aially purified dextransucrase on sucrose. The proportion of D-glucosyl residues substituted at C-3 is diminished in this case. It is concluded that several enzymes are involved in the dextran synthesis. |
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