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Somatic embryogenesis from cell suspension cultures in Carica candamarcensis
Authors:Miguel Jordan
Institution:(1) Laboratorío de Botánica, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Casilla 114-D, Santiago, Chile
Abstract:Cell suspension cultures of Carica candamarcensis derived from hypocotyl calli were tested concerning their in vitro embryogenic capacity to improve asexual propagation rates in this species. Somatic embryos developed in culture from cells in suspension or from microcalli. Responses were affected by nutrient media and phytohormones used. Best results were obtained by growing the cells in suspension in Nitsch and Nitsch medium containing naphthaleneacetic acid and then plating them upon the same medium containing benzyladenine, or combinations of both hormones.
Keywords:Carica candamarcensis  papaya  somatic embryogenesis  cell suspension cultures
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