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A Comparative Electron Microscopic Study of the Morphology of Toxoplasma gondii by Freeze-Etch Replication and Thin Sectioning Technic
Authors:ALEXANDER J. SULZER  PHYLLIS L. STROBEL  E. LOUISE SPRINGER  IVAN L. ROTH  CAREY S. CALLAWAY
Affiliation:Parasitology Division, Center for Disease Control, Public Health Service, U.S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333.;Pathology Division, Center for Disease Control, Public Health Service, U.S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333.;Department of Medical Microbiology, School of Public Health, University of California, Berkeley, California.;Microbiology Department, University of Georgia, Athens, Georgia.
Abstract:SYNOPSIS. Freeze-etch preparations of Toxoplasma gondii reveal details of structure and organelles in 3-dimensional relationships. The subpellicular microtubules and their relationship to the polar ring, the tripartite pellicle, the pellicle constituents, and the spatial relationship of the rhoptries to the conoid and conoid canal are clearly demarcated.
Keywords:Toxoplasma gondii    morphology    freeze-etching    electron microscopy
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