Engineering of a bioluminescent antigen-binding protein |
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Authors: | P. Billiald M. Mousli M. Goyffon D. Vaux |
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Affiliation: | (1) Faculté de Pharmacie, 37200 Tours (F), France;(2) Muséum national dHistoire naturelle, L.E.R.A.I., 57 rue Cuvier, 75005 Paris (F), France;(3) Sir William Dunn School, University of Oxford, South Parks Road, OX1 3RE Oxford, UK |
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Abstract: | A gene fusion plasmid was constructed by inserting the structural gene of the firefly luciferase into a vector which allows expression of a functional recombinant scFv. The fusion protein which is expressed in the periplasm of bacteria, retains dual activity and functions in bioluminescent immunoassays. Such a genetic approach offers many advantages over chemical cross-linking of antibodies to colorimetric enzymes and may be adaptable to clinical immuno-assays and high through-put drug screening assays. |
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