Chicken egg yolk-supplemented medium and the serum-free growth of normal mammalian cells |
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| Authors: | D. K. Fujii D. Gospodarowicz |
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| Affiliation: | (1) Cancer Research Institute, University of California, San Francisco, 94143 San Francisco, California;(2) Departments of Medicine and Ophthalmology, University of California, San Francisco, 94143 San Franscisco, California;(3) Present address: Reproductive Endocrinology Center HSW 1656. Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, 94143 San Francisco, CA |
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| Abstract: | Summary Supplementation of tissue culture medium with chicken egg yolk can support the proliferation of low density bovine vascular and corneal endothelial cells and vascular smooth muscle cells maintained on basement lamina-coated dishes. The optimal growth-promoting effect was observed at concentrations of 7.5 to 10% egg yolk (vol/vol). The average doubling time of bovinn vascular endothelial cells during their logarithmic growth phase when exposed to egg yolk-supplemented medium was longer than that of their counterparts grown in serum-supplemented medium (21 versus 15 h, respectively). Cultures grown in egg yolk-supplemented medium on basement lamina-coated dishes could be serially passaged, but their in vitro life span (15 generations) was less than that of serum-grown cultures (50 generations). The egg white was devoid of any grwoth-promoting activity. This work was supported by Grants HL 20197 and HL 23678 from the National Institutes of Health, Bethesda, MD. |
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| Keywords: | chicken egg yolk basement lamina extracellular matrix serum-free media vascular endothelium phospholipids |
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