Development of an Argopecten-Specific 18S rRNA Targeted Genetic Probe |
| |
Authors: | Marc E Frischer Jean M Danforth Libby C Tyner Jay R Leverone Dan C Marelli William S Arnold Norman J Blake |
| |
Institution: | (1) Skidaway Institute of Oceanography, 10 Ocean Science Circle, Savannah, GA 31411, U.S.A., US;(2) Florida Department of Environmental Protection, St. Petersburg, FL 33701, U.S.A., US;(3) University of South Florida, Department of Marine Science, St. Petersburg, FL 33701, U.S.A., US |
| |
Abstract: | Comparison of 18S ribosomal RNA gene sequences between diverse bivalve species, including eight scallop species, allowed
the design of an 18S rRNA targeted oligonucleotide probe (BS-1364) that was specific for scallops belonging to the genus Argopecten (bay and calico scallops). The high sequence similarity of the 18S rRNA gene between Argopecten irradians and Argopecten gibbus (98.8%) prevented the design of an A. irradians species-specific probe. Hybridization studies using amplified 18S rDNA from a diverse collection of bivalve species demonstrated
that the specificity of the digoxygenin-labeled probe was consistent with the predicted specificity indicated by sequence
comparison. Hybridization studies using laboratory-spawned bay scallop veligers indicated that a single veliger could be detected
by probe hybridization in a blot format, and that probe hybridization signal was proportional (r
2= .99) to the abundance of veligers. Methods for rRNA extraction and blotting were developed that allowed bay scallop veligers
to be specifically and quantitatively identified in natural plankton samples. Preliminary studies conducted in Tampa Bay,
Florida, suggest that introduced scallops can successfully spawn and produce veligers under in situ conditions. The Argopecten-specific probe and methods developed in this study provide the means to study the production and fate of bay scallop larvae
in nature and provide evidence that scallops introduced into Tampa Bay have the potential for successful reproduction and
enhancement of scallop stocks.
Received January 25, 1999; accepted May 7, 1999. |
| |
Keywords: | : 18S rRNA probe bay scallop veliger Tampa Bay Florida |
本文献已被 PubMed SpringerLink 等数据库收录! |
|