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<Emphasis Type="Italic">Enterobacter</Emphasis> spp.: A new evidence causing bacterial wilt on mulberry
Authors:Bo Zhu  GuoFen Wang  GuanLin Xie  Qin Zhou  Min Zhao  Kawicha Praphat  Bin Li  WenXiao Tian
Institution:(1) State Key Laboratory of Rice Biology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, P. O. Box 3-398, Hangzhou 310029 Zhejiang province, People’s Republic of China;(2) Institute of Environmental and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737 Hainan province, People’s Republic of China;(3) Agricultural Bioresource Research Institute, Fujian Academy of Agricultural Science, Fuzhou 350003 Fujian province, People’s Republic of China;(4) Faculty of Natural Resources and Agro-Industry Kasetsart University, Sakonnakhon Province, 47000, Thailand;(5) USDA-ARS-USHRL, Fort Pierce, FL 34945, USA;
Abstract:Thirty-six pathogenetic bacterial strains were isolated from wilted mulberry plants in Hangzhou, Zhejiang province of China. The six representative strains were confirmed to be involved in more than one Enterobacter species by common bacteriological test, electron microscope observation, hypersensitive reaction, Koch’s postulates, physiological and biochemical test, biolog, fatty acid methyl esters analysis (FAMEs), enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR), 16s rRNA sequences analysis, and comparative analysis with 7 type strains and 3 reference strains. This is the first report on mulberry disease caused by Enterobacter spp. in the world providing new evidence on induction of the plant disease in this genus. The results are not only important in the mulberry disease management but also have significant scientific value for further studies of opportunistic human pathogens and environmental strains in Enterobacter.
Keywords:mulberry enterobacter wilting  Enterobacter  phenotype identification  ERIC-PCR  16S rRNA
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