哺乳动物细胞表达系统及其研究进展

从表达载体、宿主细胞、表达系统的类型及选择等四个方面对哺乳动物细胞系统进行了综述。对相关的研究进展，如高效启动子的发现和改构、新型细胞株的建立及诱导表达调控系统亦作了简要介绍。通过这些阐述，期望能建立一个简洁而清晰的有关该表达系统的框架，对在实验中决定选取何种载体、细胞株或何种表达方式时能够有所提示。

Mammalian expression systems and its progresses

Mammalian cell expression system has been essential to the production of recombinant proteins as well as tools in research on protein structure and function.We here make a mini review on this expression system from four aspects,including expression vector,cell host,type and choice of expression system.Some related progresses are al-so discussed here,such as induced expression system,newly developed strong promoters and engineered cell lines for high protein production.By this elucidation,we hope to be able to make a simple and clear profile on the ex-pression system,which maybe helpful in deciding to select which type of promoter,vector,cell host and expression mode for a gene expression experiment.