Mechanisms of spontaneous mutation in DNA repair-proficient Escherichia coli |
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Authors: | Jennifer A Halliday Barry W Glickman |
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Institution: | Department of Biology, York University, Toronto, Ont. M3J 1P3, Canada |
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Abstract: | This paper describes the DNA sequence analysis of 729 independent spontaneous lacI? mutation This total is comprised of 478 novel mutations and 251 previously described events, and therefore should allow a more comprehensive view of spontaneous mutation in Escherichia coli. The spectrum is dominated by a hotspot (71% of all events). Mutations at this site consist of related addition and deletion events involving a number of repetitive sequences. Here we discuss how the frequency and proportion of these events vary in different DNA repair-deficient genetic backgrounds. The distribution of non-hotspot events includes base substitutions (38%), deletions (35%), frameshifts (14%), duplications (4%) and insertion elements (4%). G:C → A:T events dominate among base substitutions, while G:C → C:G events are the least common; the remaining types of base substitution are equally represented. Among deletions, a significant number do not display repeated sequences at their endpoints (26/72). However, almost all multiply recovered events (15/17) possess repeated sequences capable of accounting for the deletion endpoints. Similarily, over of all duplications recovered (5/7) display repeated endpoints. Single-base frameshifts are equally divided between A:T and G:C sites, in each case (?) 1 events occur 3-fold more frequently that (+)1 events. A comparative analysis of each mutational class recovered to lacI? spectra available in a variety of DNA repair/metabolism-deficient strains is presented here in an attempt to assess possible contributions from chemical, physical and enzymic sources of damage. |
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Keywords: | LacI Mechanisms of mutation DNA repair DNA sequences |
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