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Ouabain sensitivity is linked to ras -transformation in human HOS cells
Authors:L E Benade  N Talbot  P Tagliaferri  C Hardy  J Card  M Noda  N Najam  R H Bassin
Institution:1. ESTeam Paris Sud, INGESTEM National IPSC Infrastructure, UPSud, Paris-Saclay University, Villejuif 94800, France;2. UMR-S 935, INSERM, University Paris Sud, Villejuif 94800, France;3. Gustave Roussy Institut, Department of Biopathology, Villejuif 94800, France;4. Paris Descartes University, Faculty Sorbonne Paris Cité, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, France;5. CNRS-UMR8199, Institut Pasteur de Lille, Lille, France;6. Lille University, Lille, France;7. Department of Genomics of Common Disease, School of Public Health, Imperial College London, Hammersmith Hospital, London, United Kingdom;8. Division of Hematology, APHP-Paris Sud University, Le Kremlin Bicêtre 94275, Villejuif 94800, France;9. Paris Sud University, UPsud, Faculty of Medicine, Le Kremlin Bicêtre 94275, France;1. Central Research Institute for the Molecular Pathomechanisms of Epilepsy, Fukuoka University, Japan;2. Department of Physiology, Keio University School of Medicine, Japan;3. Department of Pediatrics, Jikei University School of Medicine, Japan;4. Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Japan;5. Department of Neurology, The University of Tokyo Hospital, Japan;6. Medical Genome Center, The University of Tokyo Hospital, Japan;7. Department of Pediatrics, School of Medicine, Fukuoka University, Japan
Abstract:Mouse cells transformed by the retroviral oncogene v-Ki- ras are significantly more sensitive to the toxic effects of 1mM ouabain than are their nontransformed counterparts. We have extended these findings to a human cell line (HOS). HOS cells (ATCC CRL 1543) are relatively resistant to treatment with 1 microM ouabain while KHOS cells (transformed by Kirsten murine sarcoma virus) are extremely sensitive. Two flat revertant cell lines isolated from the KHOS line and lacking the v- ras gene sequences are resistant to ouabain. This effect may be observed morphologically and can also be demonstrated by dye exclusion and plating efficiency tests. In addition, the toxic effects of ouabain may be rapidly and efficiently quantitated in a 51Cr-release assay. This differential lethality may be used to enrich the proportion of non-transformed revertants in populations of mutagen-treated transformed cells.
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