Variation in endoplasmic-reticulum-associated glycoproteins of carrot cells cultured in vitro |
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Authors: | Jian Guo Dong Shinobu Satoh Tadashi Fujii |
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Affiliation: | (1) Institute of Biological Sciences, University of Tsukuba, Sakura-mura, 305 Ibaraki, Japan |
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Abstract: | Glycoproteins extracted from microsomes of in-vitro-cultured cells of Daucus carota L. cv. US-Harumakigosun were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and detected by peroxidase-conjugated concanavalin A. The appearance of a glycoprotein with Mr 31 000 (GP 31) was correlated with the ability of cells to form somatic embryos. GP 31 appeared in embryogenic cells cultured in 2,4-dichlorophenoxyacetic acid (2,4-D)-containing medium, but not in somatic embryos and non-embryogenic cells; it disappeared when the cultures were transferred to auxin-free medium. Another glycoprotein with Mr 32 000 (GP 32) was detected only in non-embryogenic cells, regardless of the presence or absence of 2,4-D. Both glycoproteins, GP 31 and GP 32, were associated with the rough endoplasmic reticulum and were extractable with 0.05% deoxycholate.Abbreviations Con A concanavalin A - 2,4-D 2,4-dichlorophenoxyacetic acid - ER endoplasmic reticulum - GP 31, GP 32 a glycoprotein with an apparent molecular mass of 31 or 32 kdalton - kDa kilodalton - MS Murashige and Skoog - Mr apparent molecular mass - SDS-PAGE sodium dodecylsulfate-polyacrylamide gel electrophoresis |
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Keywords: | Cell culture (glycoproteins) Concanavalin A Daucus Embryogenesis (somatic) Endoplasmic reticulum Glycoprotein Somatic embryogenesis |
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