Identification of phytoplasmas associated with sesame phyllody disease in southeastern Iran |
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| Authors: | Susan Asghari Tazehkand Jahangir Heydarnejad Heshmat Rahimian Hossain Massumi |
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| Institution: | 1. Plant Protection Management, Western Azerbaijan Province, Urmia, Iran;2. Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran;3. Department of Plant Protection, University of Agriculture Sciences and Natural Resources, Sari, Iran |
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| Abstract: | Phyllody disease is a threat to sesame production in Kerman province, southeastern Iran. RFLP analysis of PCR products of phytoplasma-specific 16S rRNA gene (1.8 kb) and phylogenetic analyses of 16S-23S rDNA spacer region (SR) sequence indicated that the predominant agent associated with sesame phyllody in Kerman province is a phytoplasma with 100% similarity with eggplant big bud, and peanut witches’-broom phytoplasmas, members of “Candidatus Phytoplasma aurantifolia” from Iran and China, respectively. Among the samples tested, only one strain (SPhSr1), had a unique RFLP profile and its SR was 100% similar in nucleotide sequence with the phytoplasma carried by Orosius albicinctus and Helianthus annus witches’-broom phytoplasma from Iran, members of “Ca. Phytoplasma trifolii”. Virtual RFLP patterns of SPhJ2 (representative of the predominant PCR-RFLP profiles) SR sequence were identical to those of peanut witches’-broom phytoplasma (16SrII-A, JX871467). However, SPhSr1 SR sequence patterns resemble (99.7%) those of vinca virescence phytoplasma (16SrVI-A, AY500817). |
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| Keywords: | In silico RFLP phytoplasma sesame 16S-23S rRNA Iran |
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