Conserved and novel miRNAs in the legume Phaseolus vulgaris in response to stress |
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Authors: | Catalina Arenas-Huertero Beatriz Pérez Fernando Rabanal Daniel Blanco-Melo Carlos De la Rosa Georgina Estrada-Navarrete Federico Sanchez Alejandra Alicia Covarrubias José Luis Reyes |
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Affiliation: | (1) Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, 62210 Cuernavaca, Morelos, Mexico |
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Abstract: | MicroRNAs (miRNAs) are small RNA molecules recognized as important regulators of gene expression. Although plant miRNAs have been extensively studied in model systems, less is known in other plants with limited genome sequence data. We are interested in the identification of miRNAs in Phaseolus vulgaris (common bean) to uncover different plant strategies to cope with adverse conditions and because of its relevance as a crop in developing countries. Here we present the identification of conserved and candidate novel miRNAs in P. vulgaris present in different organs and growth conditions, including drought, abscisic acid treatment, and Rhizobium infection. We also identified cDNA sequences in public databases that represent the corresponding miRNA precursors. In addition, we predicted and validated target mRNAs amongst reported EST and cDNAs for P. vulgaris. We propose that the novel miRNAs present in common bean and other legumes, are involved in regulation of legume-specific processes including adaptation to diverse external cues. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | miRNAs Water deficit Glycine max Medicago truncatula Common bean Abscisic acid |
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