Conversion of hexadecanoic acid to hexadecenoic acid by rat Delta 6-desaturase |
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Authors: | Guillou Hervé Rioux Vincent Catheline Daniel Thibault Jean-Nöel Bouriel Monique Jan Sophie D'Andrea Sabine Legrand Philippe |
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Affiliation: | Laboratoire de Biochimie, INRA-ENSA, Rennes, France. |
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Abstract: | A higher content of C16:1 n-10 has recently been reported in the preputial gland of mice with a targeted disruption of the gene encoding stearoyl-CoA desaturase 1 (SCD1-/- mice) when compared with wild-type mice. This result has provided the first physiological evidence for the presence and regulation of a palmitoyl-CoA Delta 6-desaturase in mammals. To investigate the putative involvement of the known Delta 6-desaturase (FADS2) in this process, COS-7 cells expressing rat Delta 6-desaturase were incubated with C16:0. Transfected cells were able to synthesize C16:1 n-10, while nontransfected cells did not produce any C16:1 n-10. Evidence is therefore presented that the rat Delta 6-desaturase, which acts on the 18- and 24-carbon fatty acids of the n-6 and n-3 series, is also able to catalyze palmitic acid Delta 6 -desaturation. |
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